Effect of different stress conditions on the vegetative growth of the filamentous fungus Acremonium chrysogenum.

Sandra Milena Aldana Bohórquez, Ramón Ovidio García-Rico

Resumen


El hongo filamentoso Acremonium chrysogenum produce una mezcla de sustancias antibióticas, entre las que destaca la cefalosporina C. Con una participación de mercado del 50% para la cefalosporina C y sus derivados semisintéticos, desempeñan un importante rol en la industria farmacéutica. Debido a su amplio espectro de efectividad contra las bacterias Gram (+) y Gram (-), la cefalosporina C es un fármaco importante, producido únicamente por A. chrysogenum. Las condiciones ambientales inducen adaptaciones en los seres vivos, que responden modificando sus procesos biológicos para lograr su supervivencia. Estas respuestas pueden evaluarse in vitro para dilucidar el efecto del estrés fisiológico en el desarrollo. De esta manera, en este trabajo se evaluó el efecto de diferentes osmolitos, del peróxido de hidrógeno y la luz blanca sobre el crecimiento vegetativo de A. chrysogenum. Se observó un incremento del crecimiento de A. chrysogenum en condiciones de NaCl 0,5 M, mientras que frente al KCl  fue osmosensible, deduciéndose una osmoadaptación al NaCl. El glicerol solamente mostró efectos inhibidores del crecimiento a concentraciones de 1M. Por otro lado, A. chrysogenum presentó tolerancia al estrés oxidativo inducido por el peróxido, incluso a concentraciones de 100 mM. Finalmente, un fotoperíodo LD (12/12) estimuló el desarrollo del hongo, mientras que en condiciones LL la tasa de crecimiento fue similar a la observada en la condición de control (DD).

Palabras clave: Acremonium chrysogenum, estrés osmótico, estrés oxidativo, estrés lumínico.

 


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Referencias


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DOI: https://doi.org/10.24054/01204211.v2.n2.2019.3535

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