CARACTERIZACIÓN DE Pasteurella multocida subsp multocida EN LA NEUMONÍA PORCINA
Resumen
A partir de 181 muestras de pulmones con lesiones de neumonía de cerdos recolectadas en el matadero de León durante los años 2003-2004, y de otras 76 cepas aisladas previamente durante 1987-1988; procedentes de diversas provincias de Castilla y León, se logró aislar y caracterizar fenotípica y genotípicamente un total de 221 cepas bacterianas como Pasteurella multocida. Mediante la identificación bioquímica estas cepas se sub-tipificaron como subespecie multocida. Los tipos capsulares A y D fueron determinados mediante la test de sensibilidad a la hialuronidasa y de aglutinación de la acriflavina, respectivamente. En la caracterización genotípica a nivel de especie se amplificó mediante PCR la secuencia génica KMT1, e igualmente la diferenciación de los tipos capsulares (A, B, D, E y F) se realizó mediante el análisis de la región 2 del cromosoma, de esta forma, los resultados obtenidos permitieron corroborar la caracterización fenotípica previamente realizada.
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DOI: https://doi.org/10.24054/16927125.v1.n1.2015.1462
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